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Jessica R Grant
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1

Workflow for the selection of the optimal qPCR assay:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.g001.  , 2023
Link: https://doi.org/10.1371/..
 
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2

Optimized strategy for real-time qPCR detection of Onchocer..:

Mary Doherty ; Jessica R Grant ; Nils Pilotte...
https://doi.org/10.1371/journal.pntd.0011815.  , 2023
Link: https://doi.org/10.1371/..
 
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3

Detailed protocols for DNA isolation from pools of blackfly..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s001.  , 2023
Link: https://doi.org/10.1371/..
 
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4

Mean Cq values of three qPCR assays on spiked blackfly pool:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t005.  , 2023
Link: https://doi.org/10.1371/..
 
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5

Shelf-stable reagents/polymerases compared in O . volvulus ..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t002.  , 2023
Link: https://doi.org/10.1371/..
 
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6

Comparison of results obtained for detection of O. volvulus..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s004.  , 2023
Link: https://doi.org/10.1371/..
 
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7

Comparison of OvND5 and NIH O150 qPCR performance in detect..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t008.  , 2023
Link: https://doi.org/10.1371/..
 
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8

Limit of detection for the three O . volvulus qPCR assays:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t003.  , 2023
Link: https://doi.org/10.1371/..
 
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9

Detailed protocol for preparing and testing shelf-stable qP..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s002.  , 2023
Link: https://doi.org/10.1371/..
 
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10

Calculation of assay efficiency:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.g002.  , 2023
Link: https://doi.org/10.1371/..
 
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11

Comparison of OvND5 and NIH O150 qPCR performance in detect..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s005.  , 2023
Link: https://doi.org/10.1371/..
 
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12

Mean Cq of six shelf-stable assays tested over time by the ..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t006.  , 2023
Link: https://doi.org/10.1371/..
 
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13

Comparison of OvND5 and NIH O150 qPCR results from the 129 ..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.t009.  , 2023
Link: https://doi.org/10.1371/..
 
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14

Efficiency Assay Cq values, calculations, plot and data:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s006.  , 2023
Link: https://doi.org/10.1371/..
 
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15

Cq values confirmatory assay to test for species specificit..:

Mary Doherty ; Jessica R. Grant ; Nils Pilotte...
doi:10.1371/journal.pntd.0011815.s003.  , 2023
Link: https://doi.org/10.1371/..
 
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