Zhao, Qiuchen ; Yu, Zhenyang ; Zhang, Shengyuan... ScRNA-seq data from BALF of COVID-19 patients used in all studies except in Figure S3 can be accessed in Gene Expression Omnibus under the accession number GSE145926 and GSM3660650. Data from paired PBMCs can be acquired in the Genome Sequence Archive (GSA) under accession number HRA000297. For data from BALF, we removed cells with gene number less than 200 or large than 6,000. Cells with unique molecular identifier (UMI) count less than 1,000 or mitochondrial gene percentage more than 10% (15% for PBMC) were also removed in the study. All samples were integrated with Seurat (version 3.2.2) to avoid the influence of batch effects. We utilized first 50 dimensions of canonical correlation analysis (CCA) and principal component analysis (PCA) for further analysis. ScRNA-seq data from BALF of another cohort of COVID-19 patients analyzed in Figure S3 can be accessed in the EGA European Genome-Phenome Archive database under the accession number EGAS00001004717. We removed cells ....
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2022
