Silva, Maria Bárbara The increasingly small population quantity of jaguars, associated with their ecological importance, has resulted in the development of strategies that promote their conservation. In this sense, somatic cells derived from the skin of these animals can be used for this purpose, either in the production of clone embryos, in obtaining cells induced to pluripotency and in genetic studies of the species. Thus, the proper establishment of these samples is an initial step for these applications. Therefore, the aim of the present study was to assess the damage caused by in vitro culture and cryopreservation conditions on the establishment of five fibroblast lines derived from adult jaguars. Thus, fragments of skin from the apical auricular region of one female and four males were cultured in vitro to obtain somatic cells. Initially, to identify the cell type, cells were confirmed as fibroblasts after morphological and immunofluorescence analysis, and the five strains (one animal/on....
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2022
Silva, Maria Bárbara SILVA, Maria Bárbara. Avaliação das condições de cultivo e criopreservação sobre o estabelecimento de linhagens fibroblásticas de onças-pintadas, panthera onca (linnaeus, 1758). 2020. 79 f. Dissertação (Mestrado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2020..
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2020
