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Provocative tests with Kisspeptin-10 and GnRH set the scene..:

Ludwig, Mike ; Newton, Claire ; Pieters, Ané...
Ludwig , M , Newton , C , Pieters , A , Homer , N Z M , Feng Li , X , O'Byrne , K T & Millar , R P 2022 , ' Provocative tests with Kisspeptin-10 and GnRH set the scene for determining social status and environmental impacts on reproductive capacity in male African lions (Panthera leo) ' , General and Comparative Endocrinology . https://doi.org/10.1016/j.ygcen.2022.114127.  , 2022