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Characterization of Rhizobium sp (SAR-5) isolated from root..:
Subudhi, Sachidananda
;
Sethi, Debadatta
;
Kumar Pattanayak, Sushanta
http://op.niscair.res.in/index.php/IJBB/article/view/36727. , 2020
Link:
http://op.niscair.res.in/index.php/IJBB/article/view/3..
RT Journal T1
Characterization of Rhizobium sp (SAR-5) isolated from root nodule of Acacia mangium L
UL https://suche.suub.uni-bremen.de/peid=base-ftnsdlindiaojs:oai:ojs.localhost:article_36727&Exemplar=1&LAN=DE A1 Subudhi, Sachidananda A1 Sethi, Debadatta A1 Kumar Pattanayak, Sushanta PB Indian Journal of Biochemistry and Biophysics (IJBB) YR 2020 K1 The use of efficient strain of Rhizobium is of prime importance for optimum N2 harvest through legumes. The present investigation on microsymbiont associated with root nodulation of Acacia mangium L K1 an important forest species having wider adaptability. Certain biochemical and molecular techniques were used to characterize the microsymbiont. The 16S rRNA sequence was submitted to NCBI (National Center for Biotechnology Information) with an assigned accession number as MH 661260 (SAR-5). The dendrogram revealed that the strain MH 661260 (SAR-5) was Rhizobium alamii K1 exhibiting maximum similarity with Rhizobium mesosinicum strain: NR 043548. The maximum indole acetic acid (89.00 μg/mL) was produced by metabolizing glucose followed by fructose (70.4 μg/mL) K1 mannitol (55.8 μg/mL) K1 lactose (51.4 μg/mL) K1 sucrose (46.2 μg/mL) K1 starch (39.6 μg/mL) K1 galactose (30.6 μg/mL) and maltose (26.4 μg/mL) and the least (16.5 μg/mL) was recorded in control. The plateau stage of growth was attained after 36 h of inoculation K1 but the exopolysaccharides (EPS) production was the highest (112 μg/mL) at 48 h after inoculation K1 thereafter reduced in yeast extract mineral medium. The most preferable carbon K1 nitrogen K1 and vitamin for EPS production were maltose K1 L-asparagine K1 and L-ascorbic acid K1 respectively and the least preferable were sucrose K1 KNO3 K1 and riboflavin K1 respectively. The isolate (SAR-5) could survive in the pH range of 6 to 8 and the salinity level up to 3% NaCl in laboratory conditions JF http://op.niscair.res.in/index.php/IJBB/article/view/36727 LK http://op.niscair.res.in/index.php/IJBB/article/view/36727 DO http://op.niscair.res.in/index.php/IJBB/article/view/36727 SF ELIB - SuUB Bremen
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