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Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β..:

J. David Schnettler (14310745) ; Oskar James Klein (14310748) ; Tomasz S. Kaminski (1664377)..
https://figshare.com/articles/journal_contribution/Ultrahigh-Throughput_Directed_Evolution_of_a_Metal-Free_-Hydrolase_with_a_Cys-His-Asp_Triad_into_an_Efficient_Phosphotriesterase/21792589. , 2022

Link: https://doi.org/10.1021/jacs.2c10673.s001

RT Journal
T1 <Title>Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase</Title>

UL https://suche.suub.uni-bremen.de/peid=base-ftunivfreestate:oai:figshare.com:article_21792589&Exemplar=1&LAN=DE
				
A1 J. David Schnettler (14310745)
A1 Oskar James Klein (14310748)
A1 Tomasz S. Kaminski (1664377)
A1 Pierre-Yves Colin (1310004)
A1 Florian Hollfelder (426161)
YR 2022
K1 Biophysics
K1 Biochemistry
K1 Biotechnology
K1 Evolutionary Biology
K1 Ecology
K1 Computational Biology
K1 Astronomical and Space Sciences not elsewhere classified
K1 Physical Sciences not elsewhere classified
K1 suffer suicide inhibition
K1 requires bivalent cations
K1 reached high efficiency
K1 dynamic testing ground
K1 devising new catalysts
K1 covalent adduct rather
K1 novel catalytic machineries
K1 new catalytic motif
K1 observing biocatalytic innovation
K1 focused library approach
K1 enhance mechanistic strategies
K1 intermediate formation accelerated
K1 increase p91 '
K1 hydrolyze phosphate triesters
K1 5 </ sup
K1 throughput directed evolution
K1 phosphate triesters
K1 catalytic efficiencies
K1 mechanistic solution
K1 directed evolution
K1 biocatalytic challenges
K1 k </
K1 </ sub
K1 ≈ 360
K1 ultrahigh throughput
K1 two rounds
K1 subjecting p91
K1 recent release
K1 rapidly evolved
K1 naturally evolved
K1 natural counterpart
K1 made substances
K1 hydrolysis rate
K1 free α
K1 evolutionary adaptation
K1 droplet microfluidics
K1 dependent phosphotriesterases
K1 converging onto
K1 combining focused
K1 combinatorial libraries
K1 asp triad
K1 alternative reagents
JF https://figshare.com/articles/journal_contribution/Ultrahigh-Throughput_Directed_Evolution_of_a_Metal-Free_-Hydrolase_with_a_Cys-His-Asp_Triad_into_an_Efficient_Phosphotriesterase/21792589
LK http://dx.doi.org/https://doi.org/10.1021/jacs.2c10673.s001
DO https://doi.org/10.1021/jacs.2c10673.s001
SF ELIB - SuUB Bremen

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