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Could nested PCR be applicable for the study of microbial d..:

Fan, Zhen-Yu ; Li, Xiao-Ran ; Mao, Dan-Ping...
World Journal of Microbiology and Biotechnology.  25 (2009)  8 - p. 1447-1452 , 2009
Link: https://doi.org/10.1007/s11274-009-0033-3