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A single amino acid substitution can shift the optimum pH o..:

Yasuda, T ; Takeshita, H ; Iida, R...
Biochimica et Biophysica Acta (BBA) - General Subjects.  1672 (2004)  3 - p. 174-183 , 2004
Link: https://doi.org/10.1016/j.bbagen.2004.03.012