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31P NMR spectroscopy senses the microenvironment of the 5′-..:

Schnackerz, Klaus D. ; Andi, Babak ; Cook, Paul F.
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.  1814 (2011)  11 - p. 1447-1458 , 2011
Link: https://doi.org/10.1016/j.bbapap.2011.02.001