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Energy cost for the proper ionization of active site residu..:

Hanau, S. ; Proietti d'Empaire, L. ; Montin, K....
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.  1844 (2014)  4 - p. 785-792 , 2014
Link: https://doi.org/10.1016/j.bbapap.2014.02.011