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Amiloride is a suitable fluorescent substrate for the study..:

Kawasaki, Tatsuya ; Kaneko, Chisa ; Nakanishi, Ryosuke..
Biochemical and Biophysical Research Communications.  592 (2022)  - p. 113-118 , 2022
Link: https://doi.org/10.1016/j.bbrc.2022.01.014