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Using concanavalinA as a spacer for immobilization of E. co..:

Zhuang, Meng-Yao ; Wang, Cong ; Xu, Meng-Qiu...
International Journal of Biological Macromolecules.  104 (2017)  - p. 63-69 , 2017
Link: https://doi.org/10.1016/j.ijbiomac.2017.05.150