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Immobilization of the N-terminal helix stabilizes prefusion..:

Song, Albert S. ; Poor, Taylor A. ; Abriata, Luciano A....
Proceedings of the National Academy of Sciences of the United States of America.  113 (2016)  27 - p. E3844-E3851 , 2016
Link: https://www.jstor.org/stable/26470753